HCG 5000IU — Heterodimeric glycoprotein, CAS 9002-61-3, approximate molecular weight ~36,700 Da. Supplied as lyophilized powder. Third-party tested by Janoshik Analytical. For research use only. Not for human use.
Compound Specifications
Product Details
- Compound: HCG
- CAS: 9002-61-3
- Molecular Weight: ~36,700 Da (glycosylation-dependent)
- Potency: 5,000 IU
- Purity: ≥99%
- Form: Lyophilized powder
- Testing: View COA →
- Storage: Refrigerate (2–8°C). Protect from light.
- Testing: View COA (Verified by Janoshik) →
Structural Characterization
HCG is a heterodimeric glycoprotein composed of two non-covalently associated subunits: a 92-amino acid α-subunit (common to the glycoprotein hormone family) and a 145-amino acid β-subunit. The α-subunit contains five disulfide bonds and two N-linked glycosylation sites (Asn⁵² and Asn⁷⁸). The β-subunit contains six disulfide bonds, two N-linked glycosylation sites (Asn¹³ and Asn³⁰), and four O-linked glycosylation sites in the C-terminal extension (Ser¹²¹, Ser¹²⁷, Ser¹³², Ser¹³⁸).
- Subunit Architecture: α (92 aa) + β (145 aa), non-covalent heterodimer
- Disulfide Bonds: 5 (α-subunit) + 6 (β-subunit), cystine knot motif
- Glycosylation: 4 N-linked + 4 O-linked sites; sialic acid-capped
- Molecular Formula: Glycoprotein — variable with post-translational modifications
Physicochemical Properties
- Appearance: White to off-white lyophilized powder
- Isoelectric Point: pI ~3.5 (acidic, due to sialic acid residues)
- UV Absorption: λmax 280 nm (Trp/Tyr residues)
- Solubility: Freely soluble in aqueous buffers
- Thermal Stability: Store lyophilized at 2–8°C; avoid repeated freeze-thaw cycles in solution
- SDS-PAGE: α-subunit ~22 kDa, β-subunit ~38 kDa under reducing conditions (apparent MW includes glycan mass)
FOR RESEARCH USE ONLY. NOT FOR HUMAN USE.